Fung. Sci. 20(1, 2): 27–34, 2005
The evaluation of using mushroom sawdust wastes
for cultivation of
Pleurotus citrinopileatus
Zeng-Chin Liang
1
, Chiu-Yeh Wu
2*
, and Jinn-Chyi Wang
3
1. Department of Hotel & Restaurant Management, Aletheia University, Tainan, Taiwan
2. Department of Food Science & Technology, Tung-Fang Institute of Technology, Kaohsiung, Taiwan
3. Department of Food Science & Technology, Tajen Institute of Technology, Pingtung, Taiwan
(Accepted: May 10, 2005)
ABSTRACT
In the present study, sawdust wastes of
Lentinula edodes
and
Pleurotus sajor
-
caju
were used to partially re-
place sawdust as substrate components for the cultivation of
Pleurotus citrinopileatus
, and were comparative
study on the mycelial growth and yield. The highest mycelial growth of mushroom on five different combina-
tive substrates was sawdust of
Mangifera indica
(CK), followed by sawdust of
M. indica
mixed with sawdust
waste of
P. sajor
-
caju
(2
:
1, w/w). The crop gave a maximum biological efficiency of 38.6 % on sawdust of
M.
indica
mixed with sawdust waste of
L. edodes
(2
:
1, w/w), followed by 34.1 % on sawdust of
M. indica
mixed
with sawdust waste of
P. sajor
-
caju
(2
:
1, w/w). It indicated that the substrates of sawdust of
M. indica
supple-
mented with 50% sawdust waste of
L. edodes
and of
P. sajor
-
caju
, mushroom yield increased 20% and 6%, re-
spectively, over that of sawdust of
M. indica
(CK). From the results of this study, the substrate of sawdust of
M.
indica
supplemented with 50% sawdust waste of
L. edodes
appeared to be the best substrate for growing
P.
citrinopileatus
.
Key words:
cultivation, mushroom,
Pleurotus citrinopileatus
, sawdust waste.
*
Corresponding author’s email: wuchiuyeh@mail.tf.edu.tw
Introduction
Recently, strong consumer’s demand has
stimulated increased production of
P. citrino-
pileatus
, which is the new cultivated edible
mushroom in Taiwan. Growing increase in con-
sumption of
P. citrinopileatus
is largely due to
its unique flavor and aromatic properties. Par-
ticularly, the cap of
P. citrinopileatus
is beauti-
ful golden color, which attracts many people’s
attention. Many reports indicated that this
mushroom possessed medicinal properties. For
example, some polysaccharide compounds
have been extracted from both the fruiting body
(Zhang
et al
., 1994) and mycelium (Wang
et
al
., 2005) of
P. citrinopileatus
, and found to
have antitumor activity. This mushroom also
possessed fatigue resistance, immunity enhanc-
ing, delay aging (Wang
et al
., 2001), anti-
genotoxicity (Wang
et al
., 2005), and antioxi-
dant activity (Hwang, 2003). It is significantly
appeared that this mushroom has potential for
researchers to develop its application on food
and medicinal use.
28 Fung. Sci. 20(1, 2), 2005
Pleurotus
spp. are commonly grown on a
wide range of ligninocellulosic materials
(Sa’nchez, 2004). Some experiments of the ag-
ricultural wastes studied as substrates for
Pleu-
rotus
spp. are coffee industry residues (Fan
et
al
., 2000), coffee pulp and wheat straw (Sal-
mones
et al
., 2005), waste paper (Baysal
et al
.,
2003). The substrates used in each region de-
pend on the locally available agricultural
wastes (Cohen
et al
., 2002). In Taiwan, sawdust
is the popular basal ingredient used in synthetic
formulations of substrate for producing mush-
room. Especially, some mushroom growers use
the sawdust of Mango (
Mangifera indica
),
which is one of the important fruit trees in
southern Taiwan, as the substrate for cultivation
of
Pleurotus sajor
-
caju
and
Ganoderma tsugae
.
Lentinula edodes
and
P. sajor
-
caju
are two of
the five most cultivated edible mushrooms in
Taiwan, and over 100,000 tons of these two
mushrooms sawdust wastes are generated each
year. These mushroom sawdust wastes were
partially used as organic fertilizer. However,
large volumes of sawdust wastes can be found
in the mushroom cultivated areas. These saw-
dust wastes are left to decay in the field, are
disposed of through burning or are poured into
the river. It has already become a strict impact
to forest resources and environment, and this
impact will continue to become more serious
than before. Cultivation of mushrooms on these
sawdust wastes may be one of the solutions to
transform inedible wastes into edible biomass.
Some authors attempted to evaluate the mush-
room cultivation on compost waste, which
showed the compost waste could be used to re-
cultivate other edible mushrooms such as
P.
sajor-caju
after cultivation of shiitake (Royse,
1992), and
Coprinus comatus
or
Agaricus bis-
porus
after cultivation of
Ganoderma lucidum
or
Flammulina velutipes
(Chen, 1998).
In order to decrease environmental impacts
resulting from mushroom production, this study
examines the effect of
L. edodes
and
P. sajor
-
caju
sawdust wastes on the production of
P. ci-
trinopileatus
. In the previous papers, we de-
scribed the effect of different compositions of
sawdust substrate (Liang and Wu, 2002a) and
liquid spawn (Liang and Wu, 2002b) on the
yield of
P. citrinopileatus
. Based on the above
results, five formulas of sawdust substrate,
some mixed with sawdust waste from these two
mushrooms, were used in this study.
Materials and Methods
Organism
Pleurotus citrinopileatus
(supplied by You-
Hao Mushroom Research Institute, China) was
grown on PDA at 25
°
C for regular subculture
and maintained on PDA at 4
°
C for a maximum
of 3 months.
Liquid spawn preparation
Culture conditions were basically the same
as in the previous paper (Liang and Wu,
2002b).
Pleurotus citrinopileatus
was precul-
tured on PDA at 25
°
C and mycelium was har-
vested after 7 days. Mycelial fragments from
two plates (9 cm) were transferred into 100 mL
of sterilized water and homogenized with a
waring blender. Liquid cultures were grown us-
ing 5 mL of this suspension as inoculum into
500-mL flasks containing 150 mL of medium
(20 g/L glucose, 5 g/L yeast extract, 1 g/L
KH
2
PO
4
, 0.5 g/L MgSO
4
·
7H
2
O). And the flasks
were shaken under 150 rpm at 25
°
C for 7 days
on an orbital shaker. The liquid products of the
Comments
Post a Comment